Rice nuclear marker gene
Webb24 jan. 2024 · Scientific Reports - Conversion of partial restorer Swarna into restorer by transferring fertility restorer Rf gene(s) through marker assisted back cross breeding (MABB) in rice Skip to main ... Webb20 feb. 2024 · Rice plant transformation involves introducing foreign DNA segments with a selectable marker gene (SMG) into either plant nuclear DNA or chloroplast genome [ 18 …
Rice nuclear marker gene
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Webb10 feb. 2024 · Rice protoplasts were isolated from etiolated seedlings and BEAR1–YFP fusion proteins were expressed by PEG-mediated transient expression. NLS-mCherry was used as nucleus location marker. As shown in Fig. 3 A, BEAR1 was co-localized with nucleus marker protein in rice protoplast. Webb20 maj 2011 · A first set of gene-based markers was developed based on the preliminary Pup1 gene models and published by Chin et al. (2010). The germplasm survey conducted with these markers revealed that Pup1 is largely absent from modern irrigated rice varieties but that it is highly conserved in drought-tolerant breeding lines and upland varieties.
WebbFör 1 dag sedan · We fused LAX1 with RFP as a nuclear marker, and then investigated the subcellular localization of OsbHLH067, ... Data were normalized to the rice UBQ gene, … Webb20 apr. 2024 · Most of the cloned rice blast resistance genes are classic dominant R genes. Although R genes exhibit a phenotype of high resistance or immunity to rice …
WebbGene editing vector was transformed into the wild type rice plants Nipponbare by genetic transformation mediated by Agrobacterium tumefaciens (LBA4404). Then the transformed plants were hybridized with the transgenic rice carried with hptII gene. Result: 18 plants of genetic transformation were obtained for gene editing. Webb1 mars 2011 · Genetic analysis indicated that the short root phenotype of ksr1 was controlled by a recessive mutation in a single nuclear-encoded gene. To map the ksr1 mutation, an F 2 population was generated by crossing the ksr1 mutant with Nipponbare. The KSR1 locus was linked to the SSR marker RM1223 on rice chromosome 4.
WebbIn this study, we demonstrate the targeted insertion of a 5.2kb marker-free DNA fragment at two GSHs in rice using CRISPR-Cas, and obtain homozygous carotenoid- enriched rice. Results Choosing gene insertion targets in a model rice variety.Rice (Oryza sativa) is a staple food crop for more than half of the world’s population.
WebbFör 1 dag sedan · We fused LAX1 with RFP as a nuclear marker, and then investigated the subcellular localization of OsbHLH067, ... Data were normalized to the rice UBQ gene, and values represent means ± SEM from three replicates. Significant difference (two-tailed Student’s t-test, **P < 0.01, ... tub\u0027s a2Webb1 okt. 2003 · MRG4456 is linked to the fertility restorer gene Rf5 at a distance of 1.57 cM, and another newly developed microsatellite primer, HL01, was locked at a distance of … tub\u0027s 73Webb7 dec. 2024 · To determine the subcellular localization of OsNAC45 in rice cells, GFP and a OsNAC45-GFP fusion construct were introduced into rice protoplasts with the nuclear … tub\u0027s 6cWebb31 maj 2024 · DNA methylation of three cultivars, each of the fruit tree species pear, plum and apple, was analyzed by the methylation-sensitive amplified polymorphism (MSAP) marker. All three fruit tree cultivars were found to contain apparently lower levels of methylation at the 5′-CCGG sites than all other plant species, such as rice and … tub\u0027s g8Webb20 feb. 2024 · Rice plant transformation involves introducing foreign DNA segments with a selectable marker gene (SMG) into either plant nuclear DNA or chloroplast genome [ 18 ]. Proteins encoded by SMGs confer competitive advantage on only transformed cells, making them more likely to be selected in presence of a selection agent over … tub\u0027s 1uWebbIn this study, we have used higher number of SSR markers i.e.143 in number. SSR markers will differentiate the heterozygote and homozygote individual for the target gene/QTL in the screening of progenies ... Pradhan SK. Mapping the Genomic Regions Controlling Germination Rate and Early Seedling Growth Parameters in Rice. Genes. 2024; 14(4) ... tub\u0027s 7bWebb15 nov. 2024 · As a special class of rice germplasm, wide-compatibility varieties (WCVs) guarantee the fertility of hybrids when there is cross-fertilization between two subspecies. In this study, Chenghui9348 was identified as a new member of the WCV family that improves pollen fertility in an inter-subspecific hybrid. Cytological analysis showed that … tub\u0027s a7