Phleomycin plates
Webb1 feb. 2002 · Selection on phleomycin plates gave rise to resistant fungal colonies only when transformed A. tumefaciens was used for cocultivation together with AS, indicating that induction of the vir-genes was essential for T-DNA transfer between A. tumefaciens and hyphae of ecto- mycorrhizal fungi. Webbfrom the original plate selected on 50 µg/ml of phleomy-cin maintained resistance to phleomycin. Conversely, 11 of 18 colonies selected on 100 µg/ml of phleomycin …
Phleomycin plates
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WebbPhleomycin ready made solution 0.2 mum filtered, 20mg/mL in 20mM HEPES MilliporeSigma …is a glycopeptide antibiotic produced by Streptomyces verticillus. It is a member of Bleomycin family with wide spectrum antibiotic activity in both prokaryotic and eukaryotic cells. WebbPhleomycin is a glycopeptide antibiotic and is a member of the bleomycin family. Application Phleomycin from Streptomyces verticillus has been used as an antibiotic in …
Webb26 nov. 2007 · Twice as many colonies were observed when transformants were plated on MLS plus 50 μg/ml of phleomycin (left 2 plates) versus 100 μg/ml of phleomycin (right 2 … WebbSerial dilutions of each strain were plated onto YEPD, MMS plates (0.003% and 0.004%), and phleomycin plates (3 g/ml and 6 g/ml). To measure UV sensitivity, ...
WebbTypically, 100 mm round plates are for growth/selection. ... Produced by Streptomyces verticullus and part of the structurally related group of Bleomycin/Phleomycin type … WebbPhleomycin, 2% solution in water; Signal Word. Warning; Hazard Category. Carcinogenicity Category 2; Respiratory sensitiser Category 1; Hazard Statement. H351-Suspected of …
WebbStore the drug and plates or medium containing the drug in the dark. Wear gloves, a laboratory coat, ... Phleomycin Resistance Encoded by the ble Gene from Transposon …
Webb15 juli 2014 · Phleomycin化学特性 CAS号：11006-33-0 分子式：C55H85O21N20S2Cu-HCl 分子量：1525 推荐使用浓度 大肠杆菌 Phleomycin抗性的转化子可以在低盐LB琼脂糖培养基 (yeast extract 5g/l, Tryptone 10g/l, NaCl 5g/l, Agar 15 g/l, pH 7.5)，5ug/ml Phleomycin的条件下筛选。 酵母 10 μg/ml Phleomycin 真菌 根据转化子对Phleomycin 的敏感性不同， … ethel hannaWebb10 jan. 2014 · Wild type A3-2, an A3-2 mutant carrying phleomycine resistance under control of the constitutive pcbC promoter (C), and three independent xyl1 P-phleo … firefox mail accountWebbThe phleomycin-resistant cells ( Fig. 9.6) were obtained in an average of 1/1.6 plates through selection for 3–4 weeks, with a transformation efficiency of approximately 5.5 × … ethel hamptonWebb19 sep. 2013 · The pretreated MA plates were prepared by adding either 50 or 100 µg of Phleomycin (from a 20mg/mL stock) drop wise to each plate in three different regions. After allowing the antibiotic to dry (c.a., 30 min), the individual strains were plated across an entire plate. Resistance was evaluated after two days of growth at either 25°C or 13°C. firefox make bing default searchWebb29 mars 2012 · Verification of mutant strains by drug sensitivity. (a) Phleomycin plates were incubated for 3 days at 30°C for S. cerevisiae and 5 days at 23°C for S. bayanus. firefox mail downloadWebb29 apr. 2024 · Given phleomycin causes global DSBs by an unknown mechanism, we also tested the effects of deletion of DMA1 and DMA2 on plates containing an established … firefox make secureWebb2 dec. 2010 · PDF Sectoring patterns of mutants in the rad52 epistasis group. Top panels: 5ORIΔ-ΔR chromoductants of rad50Δ, rad51Δ, rad52Δ, rad54Δ, rad55Δ, rad57Δ,... Find, … firefox maintenance service