2024 Myotube fusion index

Myotube fusion index

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August undefined, 2024

WebNov 4, 2024 · The fusion index denotes the ratio of the nuclei number in myocytes with multiple nuclei, and the maturation index is the proportion of myotubes having five or … WebMyotubules are multinucleated cells which occur from cells fusing together and are the precursor to muscle fibers. The fusion index is calculated by staining the fixed cell and …

Frontiers An Assessment of Myotube Morphology, …

WebApr 11, 2024 · In particular, in 2D experiments, an enhanced myogenesis was observed, as evidenced by an increased myotube diameter and fusion index; in addition, 3D bioartificial muscles were subjected to an electromagnetic stimulus for varying exposure times: once the myotubes were formed, the pulsed electromagnetic wave caused significantly higher … The Myotube Analyzer can be used for the analysis of fixed differentiated myoblast cultures with nuclear and MyHC staining. The app can calculate the fusion index, an already existing parameter, but also provides multiple new parameters to comprehensively describe myogenic potential in its output. sylvia aas silver city nm

Myotube Definition & Meaning Merriam-Webster Medical

WebApr 26, 2024 · Myotube fusion index was evaluated and defined as the ratio of myonuclei on myotubes containing more than two nuclei. The results are expressed as the mean ± SEM of three independent cultures. Significant differences from the means of control siRNA cultures (black bar) at p < 0.05 or p < 0.01 are indicated by single or double asterisks. WebApr 29, 2024 · Increased myoblast regenerative capacity was observed as shown by the increased number of nuclei per myotube, fusion index, and maturation index. Conclusions: Ginger extracts exerted their potency in promoting muscle regeneration as indicated by prevention of cellular senescence and promotion of myoblast regenerative capacity. WebApr 19, 2024 · High concentrations of arachidonic acid have a deleterious effect on both myoblast proliferation, myotube area, and myotube fusion. Our data demonstrates that arachidonic acid reduced proliferation by 85% and 32%, myotube area by 63% and 38% and myotube fusion index by 93% and 50% respectively (P<0.001 for all). sylvia abbeyquaye

A quick, simple and unbiased method to quantify C2C12 …

Quantification of the fusion index for the C2C12 cells on …

WebMethods: We have developed a simple method to quantify myotube formation using micrographs of Jenner-Giemsa-stained C2C12 cultures. Because myotubes are darkly … Web例文 Myotube area and fusion index were measured to quantify myotube differentiation. 筋管面積および融合指数を測定して、筋管分化を定量化した。例文 Myoblast proliferation and myotube formation are critical early events in skeletal muscle regeneration. sylvia abelWebMyotube Formation. In fact, myotube formation resulted significantly affected by both PANi concentration and nanofiber alignment according to the myosin heavy chain (MHC) … sylvettes union city nj

"WebJan 24, 2024 · Our study shows that Piezo1 is essential for terminal muscle differentiation acting on myoblast fusion, suggesting that Piezo1 deregulation may have implications in muscle aging and degenerative diseases, including muscular dystrophies and neuromuscular disorders. " - Myotube fusion index

Myotube fusion index

Myotube definition of myotube by Medical dictionary

WebJun 28, 2024 · Myotube diameter and nuclear fusion index (NFI). The effect of ex vivo human serum-conditioned media on myotube diameter and NFI in vitro. A: representative images illustrating atrophy in myotubes conditioned with serum from older males in comparison to untreated control C2C12 myotubes and myotubes treated with serum from … WebNov 4, 2024 · The fusion index denotes the ratio of the nuclei number in myocytes with multiple nuclei, and the maturation index is the proportion of myotubes having five or more nuclei in a single myotube.

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Webmyotube: [ mi″o-too´būl ] a developing skeletal muscle fiber with a centrally located nucleus. adj., adj myotu´bular. WebNational Center for Biotechnology Information

WebNational Center for Biotechnology Information WebA syncytial myotube is obtained by one FC fusing with a determined number of FCMs to generate a muscle displaying a specific number of nuclei. The fusion process occurs …

WebJun 22, 2024 · The fusion index was determined to estimate the fusion capacity of the cultured myoblasts within the different stimulation conditions. The fusion index was … WebJun 22, 2024 · The fusion index was determined to estimate the fusion capacity of the cultured myoblasts within the different stimulation conditions. The fusion index was found to be slightly increased in the 22 h per day stimulation condition (77 ± 7%) compared to statically cultured myotubes (69 ± 9%) ( Figures 2A,B ).

WebAdditionally, immunofluorescence staining of MyHC and MyoD shows that overexpression of miR-424(322)-5p reduces the number of myotubes and decreases the myotube fusion index. Consistently, inhibition of its function mediated by an inhibitor promotes the expressions of myogenic markers and myotube fusion.

WebJun 15, 2024 · To investigate the role of nuclear receptor interaction protein (NRIP) in myoblast fusion, both the primary myoblasts from muscle-specific NRIP-knockout mice and NRIP-null C2C12 cells (KO19 cells) exhibited a significant deficit in the fusion index during myogenesis; on the other hand, overexpressed NRIP in KO19 cells could rescue myotube … sylvia 49 flight attendant new yorkWebDec 20, 2024 · A fusion index demonstrated that such scDKO myoblasts exhibited a significant defect in ERKi-induced secondary fusion compared with those isolated from their WT littermates (Figures 6 E and 6F). Specifically, scDKO myoblasts exhibited a loss of the hyperfused myotubes observed in the WT cultures and instead accumulated … tft items and champsWebJun 22, 2024 · In 2D, enhanced myogenesis was observed, as evidenced by an increased myotube diameter and fusion index. Second, 2D results were translated towards 3D … sylvia 5 piece dining setWebMar 31, 2024 · The final ATAC and gene expression libraries were sequenced on an Illumina Novaseq 6000 with v1.5 Kit. For the ATAC libraries, 24 bp of cell barcode and spacer, 8 bp single index read, and 100 bp paired-end reads were generated; for the gene expression libraries, 100 bp paired-end reads and 10 + 10 index reads were generated. tft item craftingWebJan 1, 2013 · 3.1 The Effect of Taurine Treatment on the Differentiation of C2C12 Myoblast to Myotube. The effect of taurine treatment on the differentiation of myoblast to myotube in C2C12 cells was evaluated by the measurements of maximal length of short diameter of differentiated myotube, the number of nucleus in the myotube, and the calculation of … tft items lucianWebTo analyse fusion index, the nuclei of 60–90 myotubes in each well were counted to obtain the average number of nuclei per myotube. Immunostaining For immunofluorescent detection of different phenotypes of muscle satellite cells under different conditions, the following antibodies were used: rabbit polyclonal anti-MyoD (1:100, Santa Cruz ... sylvia abney coldwater msWeb例文 Myotube formation was quantified by differentiation index, fusion index, and myonuclei number per myotube (see below for detailed description). 筋管形成は、分化指数、融合指数、および筋管あたりの筋核数によって定量化されました（詳細な説明については以下を参 … sylvia abonyi