Hepg2 and tgf-beta and western blot
Web22 aug. 2013 · Western Blot analysis was not sensitive enough to confirm weak cell death (+7%) of HepG2 upon TGF-β treatment on protein level. Download: PPT. PowerPoint … Web1 dec. 2006 · Journal of Pharmacology and Experimental Therapeutics Cycloheximide (CHX), an inhibitor of protein synthesis, has been reported to prevent cell death in a wide variety of cell types and produced by different apoptotic stimuli. However, the mechanisms by which CHX protects cells from apoptosis are still unclear.
Hepg2 and tgf-beta and western blot
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Web27 mrt. 2024 · 转染后48 h，收集细胞进行转染分析，倒置荧光显微镜下观察细胞转染效率。采用Western blotting检测3组细胞CNOT7蛋白、TGF-β 1蛋白和核转录因子-κ B （nuclear factor kappa B，NF-κ B） p65蛋白的表达。采用ELISA法测定细胞培养上清液中TGF-β 1水平。 1.3 Western blotting WebHere, we evaluate the antitumor activity of the Solanum glycoalkaloid tomatine in hepatocellular carcinoma (HCC) in vitro and in vivo models. Purpose: To evaluate the anticancer effect of the...
Web27 jun. 2013 · TGF-β is an important pathophysiological pathway in the liver associated with fibrogenesis, and promoting extracellular matrix deposition in hepatic stellate cells after … WebWestern blotting results showed that TGF-β-mediated upregulation of MMP-9 and PLD1 expression was inhibited by treatment with MT-GNPs . To further investigate the promoter activity of MMP-9 and PLD1 in TGF-β-stimulated HepG2 and SK-Hep-1 cells, these cells were treated with MT extracts and MT-GNPs.
WebCholestasis occurs in different clinical circumstances and leads to severe hepatic disorders. The four-and-a-half LIM-domain protein 2 (FHL2) is a scaffolding protein that modulates multiple signal transduction pathways in a tissue- and cell context-specific manner. In this study, we aimed to gain insight into the function of FHL2 in cholestatic liver injury. WebFrom the TGF-β pathway protein levels and MTT proliferation assays, we conclude that HepG2 and SNU-449 cells have intact TGF-β signaling, that SNU-398 cells have …
Web30 jun. 2016 · TGF-β1 silencing can inhibit pERK1/2 level and induce NF-κB-dependent PUMA upregulation, so we next tested whether TGF-β1 could elevate the pERK1/2 level …
Web10 apr. 2024 · sST2 increased and promoted inflammatory damage in the viral myocarditis. A The generation of sST2 was determined using ELISA.B Western blot analyses of protein expression of sST2.C H&E stain was used to evaluate the degree and grade of cardiac inflammation.D LDH, CK-MB, and AST levels in the serum were determined using a fully … sato north americaWebTGF-beta Antibody detects recombinant TGF-β1, TGF-β2, and TGF-β3. The antibody also detects endogenous levels of the TGF-β1 precursor proteins. Species Reactivity: … should i learn angularWebTGF beta Receptor II was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (prepared using NETN buffer; 1 mg for IP, 20% of IP loaded) with ab225902 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different anti-TGF beta Receptor II antibody at 1 µg/ml. sat on the roof kicked off the mossWebYou-gui Pill ameliorates renal tubulointerstitial fibrosis via inhibition of TGF-β/Smad signaling pathway. Author links open overlay panel Li Wang a 1, Ai-li Cao a 1, Yang-Feng Chi b, Zheng-Cai Ju c, Pei-Hao Yin b, Xue-Mei Zhang d, Wen Peng a b. Show more. Add to Mendeley. Share. Cite. sat on stage sam smith snlWeb1 mrt. 2024 · For performing western blotting, HEK-293 and HepG2 cells were seeded at a density of 0.4 × 10 6 cells/ well in 6-well-plates. At 24 h, cells were transiently transfected … sat on the fence 7 wordsWebMethods: Exosomes were isolated by size-exclusion liquid chromatography and were analysed NTA, µBCA and western blot. ... ELISA measured TGF-β 1, PD-L1, IL-2 and IFN-gamma. Flow cytometry analysed PD-L1; CD24/CD44 as markers of stem cells; and apoptosis. Trastuzumab-mediated antibody-dependent cell cytotoxicity (ADCC) ... saton softwareWebThe protein expression levels of autophagy relative marker LC3 and Beclin1 were detected by Western blot. The effect of TGF-β1 on invasion ability of HepG2 cells was detected … should i learn c++