2024 Hepg2 and huh7

Hepg2 and huh7

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August undefined, 2024

Web29 jul. 2010 · HepG2 and Huh7 cell lines were maintained in minimal essential medium (MEM) supplemented with 10% fetal bovine serum (FBS) (Gibco, Invitrogen) in an … WebHepG2 and Huh7 cells were treated with DAS and nude mice were intrahepatically injected with human HCC HepG2 cells and maintained with or without DAS administration for 28 …

Role of AMPK-SREBP Signaling in Regulating Fatty Acid Binding-4 …

WebML-SCO2 was able to induce LDLR expression at a dose of 60 µg/mL in HuH7 and HepG2 cell lines. Furthermore, ML-SCO2 reduced PCSK9 secretion in a concentration-dependent manner in both cell lines. Piperitone oxide, the most abundant compound of the volatile constituent of ML-SCO2 (27% w/w), was isolated and tested for the same targets, … Web12 apr. 2024 · Hypoxia inducible gene domain family member 2A (HIGD2A) is a small protein of 106 amino acids embedded in the inner membrane of mitochondria and can be induced by exposure to hypoxia and low glucose [19, 20]. HIGD2A plays a key regulatory role in mitochondrial homeostasis. fort worth star telegram obit

Hepatocellular carcinoma and chemotherapy: the role of p53

WebComparison of p53 responses in HepG2 and Huh7 cells. (A) Schematic representation of p53, including key functional domains. HepG2 cells express functional, wild-type p53, … Web2 feb. 2015 · This study investigated the expression profiles of IL-10 gene in three human hepatoma cell lines including Huh7, HepG2, and HepG2 transfected with a plasmid … WebNonalcoholic steatohepatitis (NASH)-driven hepatocellular carcinoma (HCC) is rapidly rising and lacks effective therapies, yet the underlying mechanisms remain unclear. This study aims to determine the role of hypoxia-inducible lipid droplet associated protein (HILPDA), a selective inhibitor of intracellular lipolysis, in NASH-HCC. Methods fort worth star telegram obits recent

HIGD2A silencing impairs hepatocellular carcinoma growth via …

Generation and characterization of a stable cell line ... - Nature

WebThe aim of this study was to compare FA metabolism between HepG2 and Huh7 cells in response to culturing in different sera. Both HepG2 and Huh7 cells were grown in media … WebIn this study, we delineated part of the mechanism whereby HCV induces the synthesis of TNF-alpha in human liver cell lines HepG2 and Huh7. HepG2 transiently transfected with the full-length HCV cDNA expressed high-molecular-weight (HMW) TNF-alpha mRNAs, which were absent in the control cells. fort worth star-telegram obituariesWebFIGURE 4. Crystallographic interactions between NHE1CaMBR/Ca 2 /CaM complexes. A three-dimensional stereo view of the NHE1CaMBR/Ca 2 /CaM complex with two symmetry-related neighboring complexes (complex 1 and complex 2) is shown. The crystallographic interaction of the complex with complex 1 is mediated by the C-lobe of CaM, which is … fort worth star telegram obituaries for today

"WebMethods: HepG2 (expressing normal p53), HuH7 (expressing mutated p53) and Hep3B2.1-7 (not expressing p53) cell lines were cultivated in the presence of cisplatin, doxorubicin … " - Hepg2 and huh7

Hepg2 and huh7

RETRACTED ARTICLE: Circ-DENND4C up-regulates TCF4 …

WebFrom HBV side of view, PLC/PRF5 habours natural HBs (full-length) integration and moderate HBsAg secretion, whereas HepG2 and Huh7 cells are HBV-free. Huh7, however, compared to HepG2 cells, supports higher integration efficiency of exogenous HBV genomes, probably due to its unstable genomic feature. 2 votes0 thanks John Williams WebWe find that Huh-7 cells resemble HepG2 cells in the effects of exogenous lipids, microsomal triglyceride transfer protein (MTP)-inhibition, and proteasome inhibitors of …

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WebHepG2 and Huh7 cells were seeded in 6-well plates at a density of 3 × 10 5 cells/well, cultured for 24 h and then transfected with 0.5 μg of the constructed pmirGLO-RAF1 … Web11 apr. 2024 · WT HepG2, FUT8-KO HepG2, and Huh7 cells were inoculated into a 35 mm diameter glass-bottom dish (#3961-035, IWAKI, Iwaki, Japan) and cultured for 3 days until approximately 80% confluency was reached. After removing the culture medium, the cells were washed with 1 mL phosphate-buffered saline ...

Web24 feb. 2009 · Huh7 and HepG2 cell invasion assays were performed using Matrigel Invasion Chambers (BD Biosciences, CA, USA) and quantitated as described for cell migration across uncoated membranes. Briefly, cells were seeded at 2 × 10 6 cells/ml in serum-free DMEM + 0,1%BSA and placed into 24-wells culture plates containing DMEM … Web22 feb. 2024 · In vitro experiments were performed on Huh7.5-CYP ... MIP-2, IL-8, ICAM1, TNFα and CXCL10 were measured by RT-PCR. We found that plating HepG2.2.15 cells on stiff (25 kPa) gel ...

WebNational Center for Biotechnology Information WebHuh7.5 and HepG2 cells were stimulated with sorafenib, and inhibitors of the sphingolipid pathway and cell proliferation, viability, and concentrations of bioactive metabolites were …

Web29 jul. 2010 · HepG2 and Huh7 cell lines were maintained in minimal essential medium (MEM) supplemented with 10% fetal bovine serum (FBS) (Gibco, Invitrogen) in an incubator containing 5% CO 2 at 37 °C. For transfection, electro-transfection from Amaxa system was used as up to 50% for HepG2 and 85% for Huh7 of the efficiency was achieved (data …

Web23 jan. 2024 · To support our recent findings in HepG2 cells that the LDLR can act as a bona fide receptor for Lp(a) whose effects are sensitive to PCSK9, we ... internalization by Huh7 human hepatoma cells and by primary mouse and human hepatocytes. Overexpression of human LDLR appeared to enhance apo(a)/Lp(a) internalization in … fort worth star-telegram obituaries searchWebHepG2 and Huh7 cells were seeded in 6-well plates at a density of 3 × 10 5 cells/well, cultured for 24 h and then transfected with 0.5 μg of the constructed pmirGLO-RAF1 plasmid by Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions. dipu number twoWeb12 apr. 2024 · Briefly, HepG2, Huh7, and MHCC97H cells were infected with the lentivirus shHIGD2A or shCtrl and seeded onto Seahorse cell culture microplates at a density of … fort worth star telegram news archivesWebThe migration cell numbers of (A) HepG2 and (B) Huh7 were counted ( * P<0.05, ** P<0.01). (C) Cell migration was detected using the Transwell assays in liver cancer cells transfected with miR-99a ... fort worth star telegram obituaries today\u0027sWebBackground: The plant Trigonella foenum graecum, also known as fenugreek, has been shown to have anticancer, antidiabetic, anti-inflammatory, and antioxidant properties. In … dipu townWebWe also verified that miR-99a expressions in liver cancer cell lines (HepG2 and Huh7) were decreased in comparison with the normal liver cells (Fig. 1C) while HOXA1 expressions … fort worth star telegram obituaries searchWeb28 mrt. 2024 · LO2, Hep3B, Huh7, HepG2 cell lines and SMMC-7721 cell line were cultured in DMEM medium and RPMI 1640 medium,respectively.Both media were composed of the corresponding basal medium, 10% fetal bovine serum and 1% penicillin-streptomycin.They were cultured daily in a constant temperature incubator, and digestion and subculture … fort worth star-telegram obituaries archives