WebH2B-GFP was a gift from Geoff Wahl (Addgene plasmid # 11680 ; http://n2t.net/addgene:11680 ; RRID:Addgene_11680) For your References section: Histone-GFP fusion protein enables sensitive analysis of … WebMar 26, 1998 · The cDNA encoding human H2B was tagged at its carboxyl terminus with DNA encoding codon-optimized enhanced GFP [18] (Figure 1), and the chimeric gene was subcloned into a mammalian expression vector.The construct was introduced into the human HeLa cell line by transient transfection, and fluorescence microscopic observation …
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WebThe H2B program makes it essential for the employer to attest to the Department of Labor that it will provide a wage that is at par with and or goes beyond the highest of the … WebDec 25, 2024 · HeLa cells expressing a fluorescent histone marker (HeLa-H2B-RFP) were transfected with small interfering RNA(siRNA) sequences targeting either BAG3, HSPB8, or p62/SQSTM1, and were synchronized in mitosis (Figure 1 and Figure S1A–C). Mitotic cell rounding was visualized by staining of F-actin and α-tubulin in fixed cells. myt credit transfer
A monoastral mitotic spindle determines lineage fate and …
WebBacMam Technology. CellLight™ Histone 2B-GFP, BacMam 2.0, is a fusion construct of histone 2B and emGFP, providing accurate and specific targeting to cellular histone 2B-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 ... WebMay 26, 2024 · (A) Scheme of the generation of various cell types in the Drosophila midgut. ReDMM analysis of intestine from flies fed a control or a HFD (B-G). The ReDMM tracing method was combined with an esg-Gal4 driver. Therefore, ISCs and EBs are marked with mCD8::GFP and H2B::RFP, whereas renewed ECs and EECs are exclusively marked … WebJan 31, 2024 · c, Live-imaging of RFP-Utr and H2B-GFP in an intact embryo shows an additional example of apical domains disassembly during mitosis. d , Graph shows the number of inner-cells in the 16-cell stage ... the state new jersey