WebProteintech gfp trap antibody conjugated agarose beads Gfp Trap Antibody Conjugated Agarose Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebSep 16, 2024 · ChromoTek GFP-Trap® magnetic agarose affinity beads ( see Note 2 ). 2. Low protein binding microcentrifuge tubes (e.g., LoBind® tubes). 3. Magnetic separation rack (should fit 1.5-ml microcentrifuge tubes). 4. Table-top centrifuge (cooled, with rotors suitable for 1.5- and 2-ml microcentrifuge tubes, RCF:19,000 × g ). 5.
GFP-Trap® Magnetic Agarose Kit - bulldog-bio.com
WebThe ChromoTek GFP-Trap®Agarose consists of an anti-Green Fluorescent Protein (GFP) Nanobody (VHH), which is covalently bound to agarose beads. GFP-Trap Agarose is used to immunoprecipitate GFP-fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. Properties WebThe samples were centrifuged at 4 C for 15 min at 14,000g and the supernatant was transferred to a new tube. For mass spectrometry analysis of proteins co-puried with the PsAvh262 proteins, 5 ml of total protein extract was incubated at 4 C for 3 h with 30 ml of GFP-Trap_A beads (Chromotek, ABIN509397, Planegg-Martinsried, Germany). the thren family singers
Introduction Properties - Bulldog-Bio
WebThe ChromoTek GFP-Trap® is a ready-to-use affinity resin for immunoprecipitation (IP) of GFP-fusion proteins. GFP-Trap consists of an anti-GFP Nanobody/ V H H coupled to … WebAfter centrifugation, the supernatant was incubated with 20 µL GFP-Trap ® _A beads (Chromotek) for 2 h at 4°C. Then the purified proteins were detected using immunoblotting with anti-GFP antibody (ProteinGene, 2057) and anti-FLAG antibody (ProteinGene, 2064), respectively. Chemiluminescence signals were detected using the ChemiDoc ™ XRS+ ... WebFor the co-immunoprecipitation assay, GFP fusion proteins from cellular extracts were isolated and incubated with 20–30 μL of GFP-Trap magnetic beads (ChromoTek, Martinsried, Germany), according to the manufacturer’s instructions. setinterval in angular 12